Bruker BIFLEX IV MALDI-TOF
About the MALDI-TOF
Core Campus Mass Spectrometry Facility
Interim Coordinator - Brian Logue
Phone: 605-688-6698
Department of Chemistry and Biochemistry
Avera Health and Science Center, Room 131
Box 2202
South Dakota State University
Brookings, SD 57007
Specifications
- Mass Range: m/z 500-1,000,000
- Resolution (m/Δm): 5,000 (linear mode), 20,000 (reflectron mode)
- Accuracy: 200 ppm (linear mode), 10 ppm (reflectron mode)
- 337 nm Laser
- Ionization Source: Matrix-assisted laser desorption ionization (MALDI)
- Mass Analyzer: Time of flight (TOF)
Common Applications
- Characterization of biopolymers (proteins, protein digests and oligonucleotides)
- Organometallic complexes
- Some synthetic polymers
- Molecules that photoionize upon radiation by 337 nm laser
- Labile molecules
Publications using a MALDI-TOF
- Victor, L.; Fennell, A.; Grimplet, J. Proteomic analysis of shoot tissue during photoperiod induced growth cessation in V. riparia Michx. grapevines. Proteome Sci. 2010, 8.
- Moussaoui, W.; Jaulhac, B.; Hoffmann, A.; Kostrzewa, M. Riegel, P.; Prevost, G. Matrix assisted laser desorption ionization time-of-flight mass spectrometry identifies 90% of bacteria directly from blood vials. 2010, 16 (11), 1631-1638.
- Karamonova, L.; Junkova, P.; Mihalova, D.; Javurkova, B.; Fukal, L.; Rauch, P.; Blazkova, M. The potential of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for identification of biogroups of Cronobacter sakazakii. Rapid Commun. Mass Spectrom. 2013, 27 (3), 409-418.
- Kim, Y.; Kim, S.; Chung, H.; Jee, M.; Kim, B.; Kim, K.; Yoon, J.; Lee, H.; Kim, C.; Kim, S; Yoo, W.; Hong, S. Population genotyping hepatitis C virus by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis of short DNA fragments. Clin. Chem. 2005, 51 (7), 1123-1131.
- Bonk, T.; Humeny, A.; Gebert, J.; Sutter, C.; von Knebel Doeberitz, M.; Becker, C. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry-based detection of microsatellite instabilities in coding DNA sequences: A novel approach to identify DNA-mismatched repair-deficient cancer cells. Clin. Chem. 2003, 49 (4), 552-561.